Study cohort comparison of titers and dominant apparent affinity constants for FVIII-binding IgG1 and IgA antibodies with confirmed FVIII specificity by Mann-Whitney U tests. Presented are scatter dot blots of individual IgG1 (A) and IgA (B) titer values (dark blue and dark green dots) as well as associated dominant apparent affinity constants (K_A [M⁻¹]; light blue and light green dots) of FVIII-binding antibodies with confirmed FVIII specificity. Medians are indicated by bold horizontal lines. The vertical bars represent corresponding IQRs. Mann-Whitney U test-based cohort comparisons were performed. P values for group comparisons are indicated. Statistically significant P values are highlighted in bold and marked with asterisk(s): *P ≤ .050; **P ≤ .010.