![Apparent affinity constants of FVIII-binding antibodies with confirmed FVIII specificity assessed for individual Ig isotypes and IgG subclasses. Presented are the apparent affinity constants (KA [M−1]) of FVIII-binding antibodies with confirmed FVIII specificity found in the different study cohorts presented in Figure 1. All samples containing FVIII-binding antibodies with a titer of 1:40 (IgG1, IgG3, and IgA) respectively 1:80 (IgG2 and IgG4), for which FVIII-specificity was confirmed, were included in the analysis. (A) Patients with nsHA. (B) Patients with sHA. (C) Healthy donors. Some samples in each study cohort contained 2 populations of FVIII-binding antibodies with confirmed FVIII specificity with distinct KAs. These 2 populations present in the same sample are indicated by an open and a closed circle connected by a straight line. KAs and connecting lines highlighted in red belong to patient 58 (nsHA).](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/6/3/10.1182_bloodadvances.2021005745/5/advancesadv2021005745f2.png?Expires=1724246846&Signature=MyqBCFsVk5FnSFlmHyTC-H14BzBFcsSYvJlnStOA2Rp7UYBbuCDxE8JIud~L4DpxK10YYmfyT6V4xYJVYUFrKdNfllWW-xUiFWjp99FvGImlHN8jimohLmSjkor5asgJltyliHvic6Zr66yyMJi2QcjUFFjDVMx1QpxrTR3qIqtg-VObHaKma0QTp96di2V~P8PWqFaCW6KtWs-CYrUUwax7FedUaDcNAUpBcX5L-l-3q-tmVp2I-LsqEVwyfTCS~mTHa33mtt~IiOGvcmFdPyGji-Q26i19aSHomkqEMqXPrRX7~dxLXnmIrNZJUgJWhwBJslcCrTiBywalmyuljw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Apparent affinity constants of FVIII-binding antibodies with confirmed FVIII specificity assessed for individual Ig isotypes and IgG subclasses. Presented are the apparent affinity constants (KA [M−1]) of FVIII-binding antibodies with confirmed FVIII specificity found in the different study cohorts presented in Figure 1. All samples containing FVIII-binding antibodies with a titer of 1:40 (IgG1, IgG3, and IgA) respectively 1:80 (IgG2 and IgG4), for which FVIII-specificity was confirmed, were included in the analysis. (A) Patients with nsHA. (B) Patients with sHA. (C) Healthy donors. Some samples in each study cohort contained 2 populations of FVIII-binding antibodies with confirmed FVIII specificity with distinct KAs. These 2 populations present in the same sample are indicated by an open and a closed circle connected by a straight line. KAs and connecting lines highlighted in red belong to patient 58 (nsHA).