Correction of ATC-associated changes in coagulation parameters by ^superFVa treatment. Severe bleeding was induced by a midline laparotomy followed by liver laceration (LL) involving the removal of 75% of the left lobe of the liver (supplemental Figure 6). Control mice (No LL) underwent the same procedures except liver laceration. ^superFVa (SFVa) therapy (0.8 mg/kg) or saline control (0 mg/kg SFVa) was initiated 30 minutes after the induction of liver laceration and administered as a continuous infusion at a rate of 5 µL/min for 20 minutes. Plasma samples were collected 30 and 60 minutes after liver laceration (representing the before and after ^superFVa therapy time points). Shown are the plasma activity levels of (A) FV (n = 4-7), (B) FVIII (n = 4-5), and (C) plasma TAT levels (n = 3-5). Results are shown as mean ± SD. Statistical significance was determined by 1-way ANOVA with Dunnett’s multiple comparisons test. ****P < .0001.