Time course of bleeding and reversal of ongoing ATC by ^superFVa after liver laceration. Severe bleeding was induced by a midline laparotomy followed by liver laceration (LL) involving the removal of 75% of the left lobe of the liver (supplemental Figure 6). Control mice (No LL) underwent the same procedures except liver laceration. ^superFVa (SFVa) therapy (0.8 mg/kg) or saline control (0 mg/kg SFVa) was initiated 30 minutes after the induction of liver laceration and administered as a continuous infusion at a rate of 5 µL/min for 20 minutes. Plasma samples were collected 15 to 60 minutes after liver laceration. (A) Blood loss at 15 to 60 minutes after liver laceration (left, n = 5-7) and at 60 minutes after liver laceration with ^superFVa or vehicle administration (right, n = 4). (B) APTT at 15 to 60 minutes after liver laceration (left, n = 4-7) and at 60 minutes after liver laceration with ^superFVa or vehicle administration (right, n = 4). Results are shown as mean ± SD. Statistical significance was determined by Kruskal-Wallis 1-way ANOVA with Dunn’s multiple comparisons test. *P < .05; **P < .01; ***P < .001; ****P < .0001.