Microlyse degrades platelet-VWF strings on endothelial cells in ADAMTS13-inactivated plasma. (A) Time-lapse morphology of platelet-covered VWF strings on histamine-stimulated endothelial cells in ADAMTS13-inactivated plasma, after addition of Microlyse variants, caplacizumab*, or tPA (207 nM each). Bound platelets are highlighted in red. (B-F) Time-dependent lysis of platelet-VWF complexes. (G) Time to reach 50% breakdown of platelet-VWF complexes (ie, from treatment initiation). Data represent the mean ± SEM of 4 separate experiments. Comparisons were made by 1-way ANOVA with post hoc Dunnett's multiple comparison test.