Impaired postnatal survival and unilateral anophthalmia in line-H transgenic mice. Number and within-sex percentage of WT (white) and Erfe-overexpressing (TG, dark red) offspring, grouped according to offspring sex (M = male; F = female), from line-H breeding surviving at 3 weeks of age (A) or in utero at embryonic day E17.5-18.5 (B). Statistically significant differences from expected proportions were assessed by binomial testing (****P < .0001). (C) Examples of unilateral anophthalmia in line-H TG mice at day E17.5-18.5. Top and bottom panels display the same pup rotated to show, in TG mice, normal (bottom) or impaired (top) eye development compared with WT littermates.