High Erfe expression causes reduced body and kidney mass in line-H mice. (A) Growth curves from ages 3 to 16 weeks. Statistical significance of the effect of genotype (Gt) on data variation between body weights (BWs) of sex-matched, line-H male (M, square) and female (F, circle) WT (white symbols) mice and Erfe-overexpressing (TG, dark red symbols) littermates determined by using two-way analysis of variance are displayed to the right of their respective curves. Statistically significant differences (P < .05) between sex-matched WT and TG BWs at individual time points, determined by using the Student t test, are indicated by asterisks above or below curves for male and female mice, respectively. Decreased mass of gonadal white adipose tissue (WAT) pads (B) and kidneys (C) from line-H mice as a percentage of total BW at 6 and 16 weeks of age. Serum blood urea nitrogen in line-H mice at 6 and 16 weeks of age (D) and urinary albumin-creatinine ratio (ACR) in line-H mice at 16 weeks of age (E). Group means are indicated by blue lines. Groups at each age were compared by using 2-way analysis of variance to determine significant effects of Gt and sex on data variation and to identify interactions (Int) between these variables (P < .05 denoted in bold red).