Erfe overexpression causes dose-dependent iron accumulation and inadequate hepcidin expression. Liver non-heme iron (A-B), serum iron (C-D), serum hepcidin (E-F), and serum hepcidin relative to liver iron (G-H) levels at 6 (A,C,E,G) and 16 (B,D,F,H) weeks of age in male (M) and female (F) Erfe-overexpressing (TG) mice and WT littermate controls from line-L (white/pink), line-M (white/red), and line-H (white/dark red). The mean of each group is indicated by the blue line. For each mouse line, groups were compared by using 2-way analysis of variance to determine effects of genotype (Gt) and sex on data variation (significant differences denoted in bold red) and to identify interactions (Int) between these variables. In the event of significant Int between Gt and sex, individual groups were compared by using Šidák’s multiple comparisons test. P ≥ .05 (not significant [ns]), *P < .05, **P < .01, ****P < .0001.