Recognition of tumor specific antigens over time. Recovery of antigen-specific T cells and antigen spreading as detected by IFNγ ELISPOT assay in patients receiving TAA-T alone in responders (A-B) and nonresponders (C-D). For each figure, reactivity to the targeted antigens WT1, PRAME, and Survivin is shown in panel i and nontargeted antigens MAGE family, SSX2, and SOX2 is in panel ii.