Functional characterization of TAA-T products. (A) Tumor antigen specificity as measured by IFNγ ELISPOT assay of the 12 infused products after overnight restimulation with overlapping 15mer pepmixes of actin (irrelevant control antigen), WT1, PRAME, and Survivin. (B) Polyfunctionality as assessed by the release of IFNγ and TNFα by CD3⁺, CD4⁺, and CD8⁺ TAA-Ts in response to irrelevant antigen. Actin and TAA (PRAME) shown in a representative dot plot of TAA-T product and summarized for 6 TAA-T products (C). * and ** denote P < .01 for difference between actin and PRAME.