(A) The heatmap showing the cytogenetic pattern of patient #20. Each row represents a specific kind of cytogenetic abnormality while each column represents 1 cell. In total, 5 cytogenetic abnormalities were examined in 200 plasma cells. Cells are clustered into 5 subgroups. (B) Plausible clonal phylogeny tree for patients in (A) (C) Diagrams of identifying the historical timing and sequence of critical cytogenetic events by QM-FISH. (D) Number of cytogenetic clones at first time-point sampling. (E) Timeline of different cytogenetic events in MM. Timing on the x-axis is from early to late. (F) Kaplan-Meier curves of overall survival for patients with different sequences of 13q deletion and 1q21 gain occurrence.