Diagnostic biomarker analysis divided into subgroups for the time of onset of cGVHD on metabolomic biomarker patterns. Subjects were divided into 3 groups based on the time of onset of cGVHD, and the onset samples were compared with time-matched control. (A) The early-onset cGVHD (0-120 days post-HSCT; n = 11) samples to non-cGVHD control samples obtained at 100 ± 7 days (n = 110). (B) The intermediate-onset cGVHD (onset between 121 and 240 days post-HSCT; n = 22) samples to non-cGVHD control samples obtained at 6 months (n = 113). (C) The late-onset cGVHD (onset >240 days post-HSCT; n = 7) and to non-cGVHD control sample obtained at 12 months post-HSCT (n = 97). The identical identifying metabolic color code and selection criteria as in Figure 1 were used in this figure.