D100 risk-assignment analysis of plasma metabolites to predict the later development of cGVHD. We compared plasma samples obtained at D100 from subjects who later developed: (A) cGVHD ≥day 114 (n = 27), (B) moderate to severe cGVHD ≥day 114 to those who did not develop either late aGVHD or cGVHD. Different groups of metabolites are identified by different colors: amino acids (blue), amino acid-related metabolites (orange), biogenic amines (yellow), long-chain fatty acids (dark purple), lysophosphatidyl cholines (dark green), medium-chain fatty acids (light blue), monosaccharides (dark red), phosphatidyl cholines (red), short-chain fatty acids (bright green), sphingolipids (indigo), and organic acids (pink). Rigorous selection criteria were used, and for markers to be considered biologically relevant they must meet all 3 of the following criteria: (1) P ≤ .05; (2) ROC AUC ≥0.60; and (3) effect ratio of ≥1.3 or ≤0.75.