RIOK2 loss leads to ribosomal stalling and ribosome degradation. (A) Polysome profiles of lysates prepared from MA9 Riok2^fl/fl R-Cre^ER cells treated for 40 hours with EtOH or OHT. (B) Polysome profiles of lysates prepared from MA9 Riok2^fl/fl R-Cre^ER cells treated for 40 hours with EtOH or with 200 nM doxorubicin for 12 hours. EtOH control is the same sample as in panel A. (C) Polysome profiles of lysates prepared from MA9 Riok2^fl/fl R-Cre^ER cells treated with EtOH or OHT for 72 hours. (D) Western blot analysis for the indicated proteins of MA9 Riok2^fl/fl R-Cre^ER cells treated with EtOH for 72 hours or with OHT for 40 or 72 hours, as indicated.