SOX9 modulates cholesterol biosynthesis via DHCR24. (A) Total and free cholesterol levels were measured in DLBCL cells with high (Karpas-422 and DB) or low (OCI-LY1 and SUDHL8) levels of SOX9. A total of 1 × 10⁶ cells were used for each measurement. (B-D) Total and free cholesterol levels were measured in SOX9⁻ Karpas-422 cells and DB cells and in SOX9-overexpressing OCI-LY1 cells. The level of cholesterol ester was calculated: total cholesterol − free cholesterol content. (E) Immunoblot assay and densitometry quantitation of DHCR24 protein in SOX9⁻ Karpas-422 and DB cells. (F) Karpas-422 or DB cells were treated with triparanol for 48 hours or left untreated before they were subjected to cholesterol content measurement. (A-D, F) Data represent the mean ± SD of technical triplicates. *P < .05; **P < .01; ***P < .001; ****P < 0.0005.