SOX9 binds directly to the cholesterol biosynthesized gene DHCR24. (A) Binding profiles and peak calling records of SOX9 in the DHCR24 promoter. Significant peak (P < 3. 3 × 10⁻¹¹) is highlighted by a red box with dotted line. (B) ChIP assay validation of the SOX9 binding site in the promoter of DHCR24. The SOX9 consensus binding motif from −576 to −571 on the promoter of DHCR24 is schematically represented. (C) Luciferase reporter plasmids carrying wild-type DHCR24 promoter or DHCR24 promoter with mutated SOX9 binding site was cotransfected with pCDH-SOX9, shSOX9, or vector control lentiviral plasmids in 293T cells. Luciferase activities were measured 48 hours after transfection, and normalized against firefly luciferase activities. Schematic representation of DHCR24 FL and DHCR24 mutant luciferase reporter plasmids is shown. (C) Data represent the mean ± SD of technical triplicates. **P < .01.