SOX9 regulates expression of cholesterol biosynthesis–related genes. (A) A Venn diagram of a small subset of overlapping SOX9-responsive genes downregulated in RNA-seq and showing a SOX9 peak in ChIP-seq. Green, blue, and red overlaid region (n = 20). Genes are listed to the right. (B) KEGG analysis of SOX9-responsive genes categorized according to the top 10 signaling pathways. (C) Downregulation of the steroid biosynthesis pathway in SOX9⁻ Karpas-422 cells compared with the control, based on Gene Set Enrichment Analysis of SOX9-responsive genes. (D) Real-time PCR validation of steroid biosynthesized gene expression in SOX9⁻ Karpas-422 cells and DB cells. (E) qRT-PCR determination of steroid biosynthesized gene expression in SUDHL6 and OCI-LY1 cells transduced with pCDH-SOX9 lentivirus. (F) Real-time PCR assay determined the relative expression of steroid biosynthesized genes in DLBCL cells with high (n = 2) and low (n = 6) levels of SOX9. (D-F) Data represent the mean ± SD of technical triplicates. **P < .01; ****P < .005. KEGG, Kyoto Encyclopedia of Genes and Genomes.