Alignment of additional gene mutations for 240 patients with CEBPA^mut. (A) Co-mutations organized by categories of related genes, as labeled on the left. Patients are shown in order by CEBPA subgroup. The heat map includes all mutated genes in patients with CEBPA^bi, CEBPA^smbZIP, or CEBPA^smTAD. Each column represents one of the 240 analyzed samples. Mutations in the investigated genes are shown by black bars, light gray bars indicate WT status. (B) Frequency distribution of additional gene mutations identified in patients with CEBPA^bi, CEBPA^smbZIP, or CEBPA^smTAD mutations (frequency of at least 10% in 1 subgroup). RAS signaling including KRAS, NRAS, PTPN11, and CBLB; spliceosome, including SF3B1, SRSF2, and ZRSR2; and methylation, including DNMT3A, IDH1, IDH2, and TET2.