Evaluation of Cast-PCR compared with other assays. (A) Determination of the LOD in AS-PCR using different ratios of the MYD88^L265P MWCL1 cell line and the MYD88^WT gDNA clinical sample. (B) Comparison of Cast-PCR and ddPCR in tDNA and cfDNA samples. Cast-PCR can detect mutant copies at very low concentrations of cfDNA.