Predictive features for pediatric AML relapse detected through network comparison. (A) Heat map showing clustering of nodes and diagnosis and relapse sample groups from the local pediatric and TARGET cohorts based on their connection strength from networks, with the top 50 nodes pruned from the networks. (B) Arc diagrams representing the topmost connected node gene (highlighted in red; also referred to as a hub) and all of its connections for a given rule-based network. Each arc illustrates a connection between a hub gene and an associated gene, with the connection initially derived from a rule. Nodes are decreasingly sorted from the left by the edge connection value. The full networks are depicted in supplemental Figure 14. (C) Scatter plots with mean and standard deviation illustrating the log2-transformed, TMM-normalized expression values in diagnosis and unpaired relapse samples from the local pediatric and TARGET cohorts for NFATC4 and KATNAL2. The borders of the 3 bins, corresponding to low, medium, and high expression for the respective genes, are indicated by green grid lines. Applied statistical test, Mann-Whitney test. Supplemental Table 10C-D provides details regarding samples included in this figure.