Differential gene expression between paired diagnosis and relapse samples. (A) Volcano plots presenting DEGs with genes downregulated (log2FC<0) and upregulated (log2FC>0), respectively, at diagnosis compared with relapse samples, for adult and pediatric cases separated. Highly ranked genes are highlighted in dark gray (P < .05; |log2FC|>1) or blue (highly ranked in both the adult and pediatric R/PR AML cohorts). Visualization and underlying statistical calculations were performed by using Qlucore Omics Explorer version 3.6. (B-C) Spaghetti plots displaying gene expression data comparing patient-matched diagnosis and relapse samples for CR1 (B) and DPEP1 (C). The y-axis represents log2-transformed, TMM-normalized expression values. Applied statistical test, Wilcoxon matched-pairs test. Supplemental Table 10B provides details regarding samples included in this figure, and supplemental Table 14 provides details for all DEGs.