KAT2A regulates human CB erythroid progenitor specification and survival. (A) Proportion of KAT2Ash-transduced CB HSC and progenitors. Mean ± SEM of >7 individual sorting experiments. Two-tailed paired Student t test for significance; **P < .01. (B) Frequency of CFC efficiency in the HSC/MPP, MEP (top), GMP, and MLP (bottom) compartments transduced with KAT2Ash. Mean ± SEM of 7 individual CB samples. Two-tailed paired Student t test for significance; *P < .05. (C) qRT-PCR analysis of expression of erythroid-associated genes in KAT2Ash-transduced HSC from individual CB samples. N ≥ 3 independent experiments, mean ± SEM of gene expression relative to CTRLsh, normalized to HPRT1 housekeeping gene. Two-tailed paired Student t test for significance *P < .05, **P < .01, ***P < .001. (D) MA plot of RNA-seq gene expression analysis of differentially-expressed genes (red) in CTRLsh vs KAT2Ash transduced HSC/MPP cells. (E) Gene set enrichment analysis plot for erythroid differentiation (right) and platelet signature (left) in the RNA-seq data in panel D. Gene signature identifiers are ADDYA erythroid differentiation by HEMIN and GNATENKO platelet signature, respectively, as obtained from the UC San Diego and BROAD Institute Molecular Signatures Database (MSigDB).