Automated microscopic assessment of NET formation. MFIs were determined for DAPI (detection of nuclear swelling), PE (detection of histones), and fluorescein isothiocyanate (FITC) (detection of neutrophil elastase). Comparison of MFI pre and poststimulation with PMA for 180 minutes between age-adjusted controls (black columns) and MDS samples (gray columns). *Statistically significant difference.