Pyrro and HA130 alleviate arterial platelet thrombus formation in HHcy ApoE^−/− mice. (A-D) ApoE^−/− mice were intraperitoneally injected with 100 mg/kg Hcy or vehicle (saline), and after 2 hours, the mice were treated with pyrro (5 μmol/kg) or vehicle (DMSO; ≤1:3000/body weight). (A) ATX level in plasma were measured by ELISA. (B) Tail-bleeding time was determined. (C) Blood loss was assessed. HGB, hemoglobin. (D) Platelets were labeled with calcein-AM. Occlusion time of the mesenteric arteriole (left); representative images of FeCl₃-induced mesenteric arteriole thrombosis (right, the dotted line indicates the arterial vessel wall, original magnification, 200×). (E-G) ApoE^−/− mice were intraperitoneally injected with 100 mg/kg Hcy or vehicle (saline), and after 2 hours, the mice were treated with HA130 (2 μmol/kg) or vehicle (DMSO). Tail-bleeding time was determined (E) and blood loss was assessed (F). (G) Platelets were labeled with calcein-AM. Occlusion time of the mesenteric arteriole (left) and representative images of FeCl₃-induced mesenteric arteriole thrombosis (right, the dotted line indicates the arterial vessel wall, original magnification, 200×). All data are expressed as the mean ± SEM (n = 5). *P < .05 compared with ApoE^−/−; #P < .05 compared with ApoE^−/−+HHcy.