Gasdermin D expression in neutrophils is essential for organ dysfunction during polymicrobial sepsis. (A) Schematic representation of the cell transfer experiment in which neutrophils (1 × 10⁷ cells per mouse) or monocytes (1 × 10⁷ cells per mouse) from WT animals were transferred to Gsdmd^−/− mice via IV injection 1 hour after the mice were subjected to CLP and 12 hours after the animals were euthanized. (B) Circulating concentrations of MPO/DNA-NETs were quantified 12 hours after sepsis induction by CLP. (C-F) The plasma levels of organ injury markers and (G-I) cytokines TNF-α, IL-6, and IL-1β were determined 12 hours after sepsis induction by CLP. (B-I) The dashed lines indicate the upper and lower levels of the mediators measured in the control sham groups. The data are expressed as means ± SEM. *P < .05; 1-way ANOVA followed by Tukey’s test (B-I). The data are representative of ≥2 independent experiments, each including 4 to 6 animals per group.