Gene expression profiling in expandable neutrophil-primed progenitors and neutrophil-like cells with or without lipopolysaccharide (LPS) stimulation. (A) Principal component analysis of the gene expression in neutrophil-primed progenitors enhanced by BCL-XL (NeuPs-XL) and neutrophil-like cells from NeuPs-XL (NeuCs-XL) with or without LPS stimulation. (B) Gene ontology analysis of differentially expressed genes in NeuPs-XL with or without 300 ng/mL LPS stimulation. X-axis: -log₁₀(P value) of each term. Y-axis: significant enriched gene ontology term. (C) Gene expression of inflammatory cytokines in NeuCs-XL with or without LPS stimulations, using qPCR. Means of 3 independent experiments, with relative gene expression, normalized to GAPDH expression. Statistical significance was calculated using a Student t test. *P < .05, **P < .01, ***P < .001. (D) Gene set enrichment analysis of the NF-κB pathway in NeuCs-XL with or without LPS stimulation. (E) Phosphorylation and expression of proteins involved in NF-κB and MAPK pathway using NeuCs-XL with 15-, 30-, and 60-minute LPS stimulation or without LPS stimulation.