Stimulated NK cells in patients with B/T-ALL produce more cytokines than do their healthy counterparts. CyTOF analysis of the frequencies of PMA+ionomycin-stimulated NK cell subsets expressing GM-CSF (A), TNF-α (B), and IFN-γ (C) in BMMCs from healthy donors (n = 12) and patients with B/T-ALL (n = 12). CyTOF analysis of the frequencies of PMA+ionomycin-stimulated NK cell subsets expressing GM-CSF (D), TNF-α (E), and IFN-γ (F) in PBMCs from healthy donors (n = 10) and patients with B/T-ALL (n = 8). Representative dot plots showing the expression of GM-CSF, TNF-α, and IFN-γ in PMA+ionomycin-stimulated NK cells in BMMCs (G) and PBMCs (H) from ALL patients and healthy donors. Graphs show median ± interquartile range. Exact Pvalues were calculated using the Mann-Whitney U test. ns, not significant.