Inhibiting ROS abrogates clot formation and release of NETs under flow conditions. (A) Representative fluorescent images of whole blood treated with isotype control and heparin, or KKO and heparin plus vehicle control or DPI. Extracellular DNA (Sytox, blue), neutrophils (anti-CD15 AF594, green), and platelets (anti-CD41 V450, red). Quantification of area coverage of extracellular DNA, neutrophils, and platelets from KKO-induced thrombi with and without DPI. Scale bar, 50 µm. (B) Whole blood treated with normal human IgG (hIgG) and heparin or a representative HIT IgG and heparin plus vehicle control or DPI. Staining and quantification as described in panel A. Scale bar, 50 µm. Data expressed as mean plus or minus standard deviation (SD) (n = 3). *P < .05, **P < .01, and ****P < .0001 by 1-way ANOVA with Tukey correction for multiple comparisons.