HIT IgG induces NETosis and ROS-containing thrombi. (A) Representative confocal microscopy images from n = 3 experiments of KKO-induced thrombi in whole blood showing platelets (AP2 AF594, red), neutrophils (anti-CD15 FITC, green), ROS (CellROX, magenta), and DNA release (Hoechst 3342, blue). Scale bar, 20 µm. (B) Magnified image of dotted area in panel A showing KKO-induced ROS in neutrophils and platelets. Scale bar, 20 µm. (C) Confocal microscopy analysis of KKO-induced thrombus in microfluidics channels. 3-Dimensional reconstruction and distribution of DNA (Hoechst 3342, blue), neutrophils (anti-CD15 FITC, green), platelets (AP2, AF594, red), and ROS (CellROX deep red, magenta) in thrombus.