Combination of venetoclax and ibrutinib decreases T-cell compartment skewing and acts on BIM regulation in CLL cells from a Tcl1 mouse model (A) Quantification of the ratio of CD4/CD8 T cells in the different arms of the experiment. Means of CD4/CD8 T-cell ratio ± standard error of the mean (SEM) are presented; significant differences were determined by a 2-way analysis of variance (ANOVA) with Tukey’s multiple-comparisons test. *P < .05, **P < .01 ****P < .0001. (B) Percentages of phenotypes (naive, central memory, effector memory, and double negative) from CD4⁺ and CD8⁺ T cells based on CD44 and CD62L expression are presented as means ± SEM. Significant differences were determined by 2-way ANOVA with Tukey’s multiple-comparisons test. *P < .05; **P < .01; ***P < .001; ****P < .0001. (C) Western blot analysis of sorted Tcl1 PBMC leukemia cells at time of relapse. Actin protein is presented as the loading control.