FBXO11 tumor-derived mutants show impaired ability to induce BCL6 and SNAIL degradation. (A) Representative western blot for FBXO11 and BCL6 protein expression in HEK-293T cells transfected with BCL6 in combination with GFP, WT FBXO11, or FBXO11 mutants at the indicated time points after treatment with CHX. FBXO11 mutants were detected by anti-FBXO11 antibody, and BCL6 was detected by anti-BCL6 antibody. β-actin was used as a loading control. GFP was used for transfection efficiency. One representative experiment of 2 independently performed experiments is shown. (B) Impaired degradation of SNAIL by FBXO11 mutants. Representative western blot for FBXO11 and SNAIL protein expression in HEK-293T cells transfected with SNAIL and WT FBXO11 or the indicated FBXO11 mutants at the indicated time points after treatment with CHX. FBXO11 mutants were detected by anti-Flag antibody. β-actin was used as a loading control. One representative experiment of 2 independently performed experiments is shown. (C) Degradation kinetics of BCL6 by FBXO11 mutants. BCL6 abundance was measured from western blot gels as in (A) by ImageJ software and normalized for the GFP intensity of the corresponding lane. The ratio between the relative levels of BCL6/GFP at each time 0 was set as 100%. Data are from 1 of 2 independent experiments, each with comparable results. (D) SNAIL abundance was measured from western blot gels, as in (B), using ImageJ software and normalized for β-actin intensity of the corresponding lane. The ratio between the relative levels of SNAIL/β-actin at each time 0 was set as 100%. Data are from 1 of 2 independent experiments, each with comparable results.