ALL blasts induce SLC38A5 in HD-MSCs. (A) HD-MSCs were cultured in the absence or in the presence of RS4;11 cells, and the expression of SLC38A5 was analyzed after 72 hours. (B) SLC38A5 expression was evaluated in HD-MSCs after coculture with unrelated ALL blasts. (C) SLC38A5 expression was evaluated in ALL-MSCs after coculture with related ALL blasts. (A-C) Data were normalized for RPL-15 mRNA abundance and reported as folds of the mean value obtained in monoculture. Data are means ± SD of 7 (A) or 8 (B) or 6 (C) independent experiments performed with MSCs from 3 (A, UPN#10-15-17, supplemental Table 2B) or 4 (B, UPN#5-6-7-8, supplemental Table 2B) healthy donors or 6 patients (C, UPN#6-7-8-9-10-18). For panels B and C, blasts from 3 (UPN#1-2-3, supplemental Table 2A) or 6 (UPN#6-7-8-9-10-18) patients were used, respectively. **P < .01 (one-sample Student t test). (D) The expression of SLC38A5 was determined in the MSCs used for experiments shown in panels B and C. Data are expressed as fold-change, normalizing against the average expression of healthy donors and are means ± SD. **P < .01 (Mann-Whitney U test). For panels A, B, C, and D, in each panel different symbol identifies MSCs from an individual donor.