Characterization of iMφs and in vitro antibacterial activity of iMφs against S aureus. (A) Representative flow cytometric analysis of iMφs (gray filled: unstained; black line: respective antibody, a representative experiment of n = 3). (B-D) Phagocytosis of pHrodo red S aureus BioParticles. (B) Representative flow cytometry analysis (gray filled: iMφs without BioParticles; black line: iMφs with BioParticles after 6 hours at 37°C). (C) Frequency and (D) mean fluorescent intensity of BioParticle-positive iMφs 6 hours after incubation with pHrodo BioParticles at 37°C. (E) Growth of viable Newman S aureus bacteria in the presence and absence of iMφs. (F-J) Phagocytic and bactericidal ability of iMφs against S aureus Newman and MRSA. (F) Extracellular and (G) intra-cellular bacterial CFU after infecting cells with Newman at a MOI 1. (H) Extracellular and (I) intracellular bacterial CFU after infecting cells with Newman at a MOI 5. (J) Intracellular bacterial CFU after infecting cells with MRSA at a MOI 2. (C-J) Mean ± SD, n = 3. *P < .05; **P < .01; ***P < .001; ****P < .0001; ns, not significant, as determined by ordinary 1-way ANOVA with Dunnett’s multiple comparisons test (C,D) or Kruskal-Wallis ANOVA with Dunn’s correction analysis (F-J).