American Society of Hematology

Figure 5.

$Rab27A augments Rab3B and Rab3D activation. GTP-bound Rab3B (A) and Rab3D (B) fractions (Rab3B* and Rab3D*) were extracted by RIM2 PD in shCTRL- and shRab27A-transduced HUVEC lysates. (C) Western blot analysis of Rab27A expression in shCTRL and shRab27A. (D) Normalized Rab3B and Rab3D activity levels are plotted relative to total input levels. Dotted line indicates activity levels in shCTRL. (E) GTP-bound Rab27A fractions (Rab27A*) were extracted by Slac2-b PD in shCTRL-, shRab3B-, and shRab3D-transduced HUVEC lysates. (F) Western blot analysis of Rab3B and Rab3D expression in shCTRL, shRab3B, and shRab3D. (G) Normalized Rab27A activity levels are plotted relative to total input levels. Representative western blots are shown for 3 Slac2-b PD and 5 RIM2 PD biological replicates. Dotted line indicates activity levels in shCTRL. Statistical analysis used an unpaired Student t test comparing each condition to shCTRL. Data are shown as mean ± SEM. *P < .05; **P < .01.$

Rab27A augments Rab3B and Rab3D activation. GTP-bound Rab3B (A) and Rab3D (B) fractions (Rab3B* and Rab3D*) were extracted by RIM2 PD in shCTRL- and shRab27A-transduced HUVEC lysates. (C) Western blot analysis of Rab27A expression in shCTRL and shRab27A. (D) Normalized Rab3B and Rab3D activity levels are plotted relative to total input levels. Dotted line indicates activity levels in shCTRL. (E) GTP-bound Rab27A fractions (Rab27A*) were extracted by Slac2-b PD in shCTRL-, shRab3B-, and shRab3D-transduced HUVEC lysates. (F) Western blot analysis of Rab3B and Rab3D expression in shCTRL, shRab3B, and shRab3D. (G) Normalized Rab27A activity levels are plotted relative to total input levels. Representative western blots are shown for 3 Slac2-b PD and 5 RIM2 PD biological replicates. Dotted line indicates activity levels in shCTRL. Statistical analysis used an unpaired Student t test comparing each condition to shCTRL. Data are shown as mean ± SEM. *P < .05; **P < .01.

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