Targeting MADD to mitochondria displaces Rab27A from WPBs. HUVECs were transduced with TOMM70-EGFP (A) or TOMM70-EGFP-MADD (B) or TOMM70-EGFP-MADD-ΔDENN (C) (all shown in green) and immunostained for VWF (blue), Rab27A (red), and VE cadherin (not shown). Boxed areas are magnified on the right. Yellow arrowheads indicate Rab27A⁺and cyan arrowheads indicate Rab27A^– WPBs. Scale bars represent 10 µm. (D) Quantification of the percentage of transduced (GFP⁺) HUVECs containing Rab27A⁺ WPBs in TOMM70-EGFP (175 cells analyzed), TOMM70-EGFP-MADD (96 cells analyzed), and TOMM70-EGFP-MADD-ΔDENN (38 cells analyzed). Data are shown as mean and were analyzed by using one-way ANOVA with Dunnet’s multiple comparisons test. *P < .05.