HGAL interacts with tubulin. (A) HGAL reciprocally co-immunoprecipitated (IP) with α-tubulin in Raji cells. (B) BJAB cells were seeded on planar lipid bilayer membranes coated with anti-human IgM antibody (surrogate antigen) for 30 minutes to induce B-cell synapse formation and then were analyzed. Shown are confocal microscopy images of HGAL and tyrosinated α-tubulin co-localization at 0.5 μm from the cell surface. BCR, purple; HGAL, green; tyrosinated α-tubulin, orange. (C) Co-sedimentation of recombinant HGAL tagged with thioredoxin (TRX) with isolated microtubules. (D) HGAL-mediated inhibition of lymphoma motility is dependent on tubulin. Shown are number of cells migrating in transwell experiments in the presence of the indicated stimulants and colchicine (20 nM). Means and standard deviations of 6 independent experiments are shown. **P < .01; ****P < .0001.