HGAL expression inhibits U2932 lymphoma cell growth and metastasis and prolongs mouse survival. Mice were injected intravenously with 5 × 106 luciferase-transduced human lymphoma cells from cell line U2932. D-luciferin was injected intraperitoneally (150 mg/kg body weight) 15 minutes before acquiring images. Bioluminescence images were taken once per week. (A) Representative bioluminescent images of mice with U2932 Mock and U2932 HGAL lymphoma. (B) Tumor growth curve based on photon flux intensity (each group had 17 mice) with significant differences between the U2932 Mock and U2932 HGAL mouse cohorts (P = .0042). Mice that were not injected (background; 5 mice) served as a control for background photon flux intensity. Normalized photon flux represents tumor photon flux (luminescence intensity) at the indicated time relative to photon flux measured after cell injection on day 0 that was set to value of 100. (C) Number of individual sites with lymphoma involvement in U2932 Mock and U2932 HGAL mice (P = .0001). (D) Kaplan-Meier plots of mouse survival demonstrating significant difference between the U2932 Mock and U2932 HGAL mice (P = .0001). ****P = .0001.