ChAdOx1 nCoV-19 vaccine contains EDTA and induces inflammatory reactions in mouse edema models and vaccinated individuals. (A) 1H-NMR spectrum of ChAdOx1 nCoV-19 vaccine showed signals of sucrose, ethanol, histidine, and EDTA (∼100 µM). X-axis: NMR chemical shift signals in ppm relative to internal standard TSP; y-axis: relative signal intensity. (B) Skin edema in wild-type mice was induced by intradermal injection of 50 μL each saline, EDTA (100 μM), or ChAdOx1 nCoV-19. Evans blue dye was intravenously infused as a tracer, and after 30 minutes extravasated dye was quantified. Columns show mean ± SD, n = 41 per group. Paired 1-way ANOVA followed by Dunn’s multiple comparison test. (C) Digital PCR quantified AV DNA 30 minutes after intradermal injection of 50 μL ChAdOx1 nCoV-19 in wild-type mice. Segments give relative percentage in multiple organs of disseminated ChAdOx1 nCoV-19 AV copy numbers; n = 3 individual experiments. (D) Skin reaction 2 days after ChAdOx1 nCov-19 vaccination and following symptom resolution on day 14. D-Dimer was elevated at 4 and 6 days following ChAdOx1 nCoV-19 vaccination, with symptom resolution in following days.