Germline variant analysis flowchart for TARGET and Australian childhood AML cohorts. Germline variant curation workflow for WES and WGS data from TARGET and Australian cohorts and summary of results. PTM, premature termination mutation, includes frameshift indels, stop gain, or splice acceptor/donor site variants in tumor suppressor genes. BM, bone marrow; PB, peripheral blood; WES, whole-exome sequence; WGS, whole-genome sequencing.