Effect of inhibitors targeting the proximal steps in the complement pathway in the presence of KKO ULICs. WB was preincubated with the complement inhibitors or respective controls (ISO=isotype or CON=control) shown on the x-axis before adding KKO ULIC, and complement activation was measured by generation of C3c (A) or C5b-9 (B). Results shown are representative of 3 independent experiments. ***P < .0001 (1-way analysis of variance with Tukey’s multiple comparison test). ns, not significant.