Figure 2.
Hapln1b is necessary for the specification of HSPC from the hemogenic endothelium. (A) Gata2b, runx1, and cmyb ISH in control MO, or hapln1b MO-injected embryos (injected at 3 ng throughout, unless otherwise stated). (B) ISH expression of cmyb, in control MO–, or hapln1b MO–injected embryos. (C) Rag1 expression in the thymus after control MO or hapln1b MO injection. (D) ISH expression of kdrl in control MO– or hapln1b MO–injected embryos. Hapln1b morphants displayed 3 phenotypes: normal, mild, and severe. (E) ISH expression of mfap4, mpx, and gata1 in control MO– or hapln1b MO–injected embryos. H1b, hapln1b. (F) Imaging double-transgenic kdrl:mCherry/cmyb:GFP embryos at 36 hpf. Bar represents 50 µm. Control (ctl) MO vs hapln1b mRNA injected, P < .0001. Student unpaired t-test.

Hapln1b is necessary for the specification of HSPC from the hemogenic endothelium. (A) Gata2b, runx1, and cmyb ISH in control MO, or hapln1b MO-injected embryos (injected at 3 ng throughout, unless otherwise stated). (B) ISH expression of cmyb, in control MO–, or hapln1b MO–injected embryos. (C) Rag1 expression in the thymus after control MO or hapln1b MO injection. (D) ISH expression of kdrl in control MO– or hapln1b MO–injected embryos. Hapln1b morphants displayed 3 phenotypes: normal, mild, and severe. (E) ISH expression of mfap4, mpx, and gata1 in control MO– or hapln1b MO–injected embryos. H1b, hapln1b. (F) Imaging double-transgenic kdrl:mCherry/cmyb:GFP embryos at 36 hpf. Bar represents 50 µm. Control (ctl) MO vs hapln1b mRNA injected, P < .0001. Student unpaired t-test.

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