Pulmonary emboli in mice treated with EV derived from L3.6 cells. (A) Paraffin section of lung from a WT mouse after L3.6 EV infusion into the IVC (original magnification ×5; H&E stain). Scale bar, 500 μm. Frozen sections of lung following EV infusion into the IVC and staining for platelet GP1b (CD42c, red) or fibrin (monoclonal antibody 59D8, green) at original magnification 320 (scale bar, 100 mm). (B) At original magnification 310 (scale bar, 200 mm. (C) DAPI (blue) stains cell nuclei. (D) Number of thrombi observed in a random 10× field following infusion of L3.6 EVs into WT C57BL/6, f12^−/−, kng1^−/−, and klkb1^−/− mice. (E) Effect of preincubation of L3.6 EV with buffer alone (untreated) or CIP (treated) prior to IVC infusion on density of lung thrombi, measured per ×10× field. (F) Effect of preincubation of L3.6 EV with buffer alone (untreated) or CIP (treated) on time to death after IVC infusion. Each point represents an individual animal. Bars in graphs depict 95% confidence intervals , and comparisons between groups were performed using ANOVA and Student t tests. hpf, high-powered field; ns, not significant.