Analysis of the RBC lysates. Zebrafish (ZF) (A) and human (H) (B) RBC lysates were run on 5% native polyacrylamide gel and stained with EtBr. Arrows show a single band of nucleic acid. Double-stranded DNA size markers are indicated by “Marker.” (C) NaOH treatment of the gel-purified nucleic acid (indicated by arrow) from the zebrafish RBC lysates shows disappearance of the nucleic acid. (D) Quality of the purified RNA from zebrafish RBC lysates was checked by Omega Bioservices using a denaturing gel. Arrows indicate the purified RNA (150 nucleotides long) and the internal marker (25 nucleotides long). The standard RNA size markers are shown on the left.