CD8⁺ T-cell clonal space is occupied by fewer clones at occurrence of GVL. (A) Number of unique clonotypes (y-axis) required to occupy 25%, 50%, 75%, and 100% of the CD8⁺ repertoire is compared between patients with GVL effect (green; n = 14) and those without GVL (orange; n = 11). Clonotypes are used by decreasing abundance, identifying the minimal number of unique clonotypes required to cover each quartile of the CD8⁺ repertoire. Two time points are compared for each patient, with closed circles displaying the pre-GVL/noGVL time point and closed squares displaying the first onset of GVL/noGVL. (B) Difference in number of clones (y-axis) between pre-GVL/noGVL and GVL/noGVL (y-axis) is displayed individually for each quartile of the CD8⁺ repertoire. Statistical analysis was performed by Wilcoxon matched-pairs signed ranked test (two-tailed) (A) and Mann-Whitney test (two-tailed) (B). Black lines represent median; error bars show the interquartile range. *P < .05; **P < .01; ns, not significant.