Focusing of the CD8⁺ but not CD4⁺ or entire CD3⁺TRB repertoire is linked to GVL effect. Comparison of CD8⁺ (A), CD4⁺ (C), and entire CD3⁺ (E) TRB diversity via inverse Simpson’s index (1/D) in patients with GVL effect (green; n = 14) and those without (orange; n = 11). The graph shows the time point before occurrence of first GVL/noGVL (closed circles) and the first occurrence of GVL/noGVL (closed squares) for each patient. The higher the inverse Simpson’s value, the higher the TCR repertoire diversity. Change of CD8⁺ (B), CD4⁺ (D), and entire CD3⁺ (F) TRB diversity at first GVL occurrence and patients without GVL in percent is displayed. CD3⁺ diversity data were calculated based on the cell frequencies of CD8⁺ and CD4⁺ cells (eg, CD3⁺ 1/D was calculated as sum of CD8⁺ 1/D × frequency of CD8⁺ of CD3⁺ and CD4⁺ 1/D × frequency of CD4⁺ of CD3⁺). Statistical analysis was performed by Wilcoxon matched-pairs signed ranked test (two-tailed) (A) and Mann-Whitney test (two-tailed) (B). Black lines represent median; error bars show the interquartile range. **P < .01; ns, not significant.