Inhibition of Srebp limits NK cell cytokine training. (A-B) Representative dot plot and scatter graph showing impact of 25HC treatment in the initial 18 hours on IFN-γ production by trained mouse NK cells 6 days later. (C) Representative line graph demonstrating the percentage of target cells lysed by trained (red circles) or 25HC-treated (blue circles) mouse NK cells at different effector/target ratios. (D) Scatter graph displaying pooled data demonstrating the impact of 25HC on lysis of target cells by trained mouse NK cells at 5:1 target ratio. (E) Scatter graph showing impact of 25HC treatment in the initial 18 hours on IFN-γ production by trained human NK cells on day 7. (F-G) Representative dot plot and scatter graph showing impact of PF429242 treatment in the initial 18 hours on IFN-γ production by trained mouse NK cells. (H) Representative line graph demonstrating the percentage of target cells lysed by trained (red circles) or PF429242 treated (blue circles) mouse NK cells at different effector/target ratios. (I) Scatter graph demonstrating the impact of PF429242 treatment in the initial 18 hours on relative lysis of target cells by trained mouse NK cells at 5:1 target ratio. (J) Scatter graph showing impact of PF429242 treatment in the initial 18 hours on IFN-γ production by trained human NK cells at day 7. Data pooled from a minimum of 3 independent experiments unless otherwise stated. Statistical comparisons using Student t test. *P < .05; **P ≤ .01; ***P < .001.