Metabolic inhibition limits NK cell cytokine training. (A-B) Representative dot plot and scatter graph showing impact of 2DG treatment in the initial 18 hours on IFN-γ production by trained mouse NK cells 6 days later. (C) Representative line graph demonstrating the percentage of target cells lysed by trained (red circles) or 2DG-treated (blue circles) mouse NK cells at different effector/target ratios. (D) Scatter graph displaying pooled data demonstrating the impact of initial 2DG on relative lysis of target cells by trained mouse NK cells at 5:1 target ratio on day 7. (E) Scatter graph showing impact of 2DG treatment in the initial 18 hours on IFN-γ production by trained human NK cells at day 7. (F-G) Representative dot plot and scatter graph showing impact of oligomycin treatment in the initial 18 hours on IFN-γ production by trained mouse NK cells at day 7. (H) Representative line graph demonstrating the percentage of target cells lysed by trained (red circles) or oligomycin-treated (blue circles) mouse NK cells at different effector/target ratios. (I) Scatter graph demonstrating the impact of oligomycin on lysis of target cells by trained mouse NK cells at 5:1 target ratio. (J) Scatter graph showing impact of 2DG on IFN-γ production by trained human NK cells. Data pooled from a minimum of 5 independent experiments unless otherwise stated. Statistical comparisons using Student t test. *P < .05; **P ≤ .01.