Cytokine-trained NK cells display increased effector functions in both mice and humans. (A-B) Representative dot plot and scatter graph showing IFN-γ production in untrained or trained mouse NK cells. (C) Representative line graph demonstrating the percentage of target cells (K562) lysed by untrained (red circles) or trained mouse NK cells (blue circles) at different effector/target ratios. (D) Scatter graph displaying pooled data demonstrating the percentage of target cells lysed by untrained or trained mouse NK cells at 5:1 target ratio. (E-F) Representative dot plot and scatter graph showing percentage of IFN-γ production in untrained or trained human NK cells. (G-H) Representative histogram and scatter graph showing mean fluorescence intensity (MFI) of IFN-γ production in untrained or trained human NK cells. Data pooled from a minimum of 5 independent experiments unless otherwise stated. Statistical comparisons using Student t test. *P < .05; **P ≤ .01; ***P < .001.