DNA vs cDNA FLT3-ITD measurements in relation to clinical outcome. Comparison of (A-B) DNA and cDNA FLT3-ITD AR and (C) FLT3-ITD length measurements within the HOVON 102 and HOVON 132 AML cohorts. Shown are samples with detected FLT3-ITD on both the DNA and cDNA levels and samples that have positive FLT3-ITD lengths (thus excluding the single case with a 3-bp deletion). For length comparison, only samples for which we could confidently identify the FLT3-ITD peak are shown. Panel A shows patients with a maximum FLT3-ITD AR of 25. Panel B shows patients with a maximum FLT3-ITD AR of 2.25. Correlation coefficients are calculated by using all data from patients with FLT3-ITD on both the DNA and cDNA levels using Spearman rank correlation.