Macrophage and dendritic cell potential of DN cells. (A) Progression of EB cultures, in the 6-cytokine cocktail, from day 28 to 35 shows continued maintenance of the DN population. FACS analysis of the DN and DP population for HSC markers (CD34 and CD38) and myeloid progenitor markers (CD117 and CD123). (B) Day 40 EB cytokine 6 cultures CD11bCD14 DN and DP populations were evaluated for the expression of macrophage and dendritic cell markers CD169, CCR2, HLA-DR, CD40, and CD83 in the context of CD11c. (C) To assess the potential of the DN progenitor population to generate macrophages and DCs, sorted DN cells were expanded in either MCSF or GMCSF + IL4 and then analyzed for upregulation of CD11b and CD14 along with CD169, HLA-DR, CD40, and CD83 in the context of CD11c.